Chromogenic Endotoxin Quantification Kit for Accurate Bacterial Toxin Measurement

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Chromogenic Endotoxin Quantification Kit for Accurate Bacterial Toxin Measurement

Endotoxins, also known as lipopolysaccharides (LPS), are toxic components found in the outer membrane of Gram-negative bacteria. Accurate measurement of endotoxin levels is crucial in pharmaceutical, medical, and research applications to ensure safety and compliance. The Chromogenic Endotoxin Quant Kit provides a reliable and sensitive method for quantifying bacterial endotoxins.

Why Choose the Chromogenic Endotoxin Quant Kit?

This kit utilizes a chromogenic assay based on the Limulus Amebocyte Lysate (LAL) principle, which reacts specifically with endotoxins to produce a measurable color change. The key advantages include:

  • High Sensitivity: Detects endotoxin levels as low as 0.005 EU/mL

  • Wide Dynamic Range: Suitable for samples with varying endotoxin concentrations

  • Rapid Results: Provides quantitative measurements within 30-60 minutes

  • Simple Protocol: Easy-to-follow procedure requiring minimal sample preparation

  • Regulatory Compliance: Meets USP, EP, and JP requirements for endotoxin testing

Applications of the Chromogenic Endotoxin Quant Kit

The versatility of this kit makes it suitable for multiple applications:

  • Quality control in pharmaceutical manufacturing

  • Testing of medical devices and implants

  • Research on bacterial infections and sepsis

  • Monitoring of water and environmental samples

  • Validation of depyrogenation processes

How the Chromogenic Assay Works

The assay principle involves three key enzymatic reactions:

  1. Endotoxin activates Factor C in the LAL reagent

  2. Activated Factor C cleaves a synthetic chromogenic substrate

  3. The released chromophore is measured spectrophotometrically at 405 nm

The intensity of color development is directly proportional to the endotoxin concentration in the sample, allowing for precise quantification using a standard curve.

Kit Components and Storage

The complete kit includes:

  • Lyophilized LAL reagent

  • Chromogenic substrate

  • Endotoxin standard

  • Reconstitution buffer

  • Detailed protocol and data analysis instructions

All components should be stored at -20°C upon arrival, with the exception of the reconstitution buffer which can be stored at 2-8°C.

Conclusion

The Chromogenic Endotoxin Quant Kit offers researchers and quality control professionals a robust, sensitive, and regulatory-compliant solution for bacterial endotoxin measurement. Its chromogenic detection method provides superior accuracy compared to traditional gel-clot techniques, making it an essential tool for ensuring product safety and advancing microbiological research.

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